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Merck
CN

T8062

Anti-Tissue Inhibitor of Metalloproteinase-2, First Loop antibody produced in rabbit

~1 mg/mL, buffered aqueous glycerol solution, affinity isolated antibody

Synonym(s):

Anti-TIMP-2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01094316
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
2

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

western blot: 1:1,000 using a concentrated cell culture medium from a stimulated human cell line (M 2928)

UniProt accession no.

P16035

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TIMP2(7077)

Immunogen

synthetic peptide corresponding to the first loop of human tissue inhibitor of metalloproteinase-2

Biochem/physiol Actions

By immunoblotting against the reduced protein, the antibody reacts with bands at approximately 21 kDa. It also reacts with non-reduced TIMP-2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
114K1207
094K1413
120K1466
082K1582
052K1150

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Boris I Ratnikov et al.
Laboratory investigation; a journal of technical methods and pathology, 82(11), 1583-1590 (2002-11-14)
Gelatin zymography is the common method for examining matrix metalloproteinase-2 (MMP-2) in cells and media samples. Activation of the latent MMP-2 zymogen involves its binding to the cell surface MT1-MMP*TIMP-2 (membrane type-1 matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase-2) complex with
Sonia Métayer et al.
Biology of reproduction, 66(5), 1219-1229 (2002-04-23)
The testicular and epididymal fluids of ram, boar, and stallion were analyzed by means of one-dimensional and two-dimensional gelatin gel zymography. Five main gelatinolytic bands were revealed in the ram and at least seven were observed in the boar and

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