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T8778

Trehalase from porcine kidney

Name: Trehalase from porcine kidney
Brand: SIGMA

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym(s):

α,α-Trehalose glucohydrolase

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About This Item

CAS Number:

9025-52-9

EC Number:

3.2.1.28 (BRENDA, IUBMB)

UNSPSC Code:

12352204

NACRES:

NA.54

MDL number:

MFCD00132462

Specific activity:

≥1.0 units/mg protein

Concentration:

0.5-10.0 mg/mL protein (BCA)

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Properties

form

buffered aqueous glycerol solution

Quality Segment

200

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

Description

General description

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.

Application

Trehalase has been used:

in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
in a study to investigate growth arrest by trehalose-6-phosphate
to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Physical form

Solution in 50% glycerol containing 1% Triton^™ X-100 and 25 mM potassium phosphate, pH 6.5

Other Notes

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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