T9705
TGF-β3 active
Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
Synonym(s):
TGF-beta-3, Transforming growth factor beta-3
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About This Item
NACRES:
NA.32
UNSPSC Code:
51111800
biological source
human
recombinant
expressed in Nicotiana
assay
>97% (SDS-PAGE)
form
lyophilized
potency
≤50 ng/mL ED50
mol wt
27.2 kDa (two identical 118 amino acid polypeptide chains linked by a single disulfide bond.)
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | embryo: suitable
impurities
Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... TGFB3(7043)
General description
Recombinant human TGF-β3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor–β is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-β(TGF-β1, TGF-β2 and TGF-β 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.
Recombinant human TGF-β3 is a 27.2 kDa protein composed of two identical 118amino acid peptide chains linked by a single disulfide bond.
Application
TGF-β3 active along with growth factors BMP-6 and IGF-1 can be used for inducing in vitro chondrogenesis. The product can also be utilized for few physiological processes like- embryogenesis, tissue remodeling, and wound healing.
Biochem/physiol Actions
Transforming growth factor β-3 (TGF-β3) is a cytokine that belongs to Transforming growth factor-beta family and is widely present in various normal as well as neoplastic cells. TGF-β3 can reduce type I collagen deposition either by impeding myofibroblast differentiation which in turn inhibits collagen synthesis or by enhancing collagen degradation by MMP-9. The product can also induce ectopic mineralization in fetal mouse dental pulp cells by up regulating the osteocalcin (OCN) and type I collagen (COL I) expression.
Physical form
Lyophilized from a Tris HCl 0.05 M buffer at pH 7.4
Preparation Note
Lyophilized protein should be reconstituted in water to a concentration of 5 - 25 ng /μl. Due to the protein nature, dimmers and multimers may be observed.
Analysis Note
The biological activity of TGF-β3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation.
ED50 = 40 ng/mL
ED50 = 40 ng/mL
Other Notes
Extinction Coefficient: E0.1% = 1.72 (A280 nm)
p.I: 6.75
p.I: 6.75
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Muhetaer Huojia et al.
Development, growth & differentiation, 47(3), 141-152 (2005-04-21)
Several members of the transforming growth factor (TGF)-beta superfamily are expressed in developing teeth from the initiation stage through adulthood. Of those, TGF-beta1 regulates odontoblast differentiation and dentin extracellular matrix synthesis. However, the molecular mechanism of TGF-beta3 in dental pulp
Nitaya Indrawattana et al.
Biochemical and biophysical research communications, 320(3), 914-919 (2004-07-09)
During the last decade, many strategies for cartilage engineering have been emerging. Stem cell induction is one of the possible approaches for cartilage engineering. The mesenchymal stem cells (MSCs) with their pluripotency and availability have been demonstrated to be an
R Hosokawa et al.
Journal of dental research, 82(7), 558-564 (2003-06-25)
Cleft lip is a common congenital malformation, and labioplasty performed on infants to repair such defects often results in severe scar formation. Since TGF-beta 3 has been implicated in wound healing, we therefore hypothesized that TGF-beta 3 functions to reduce
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