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Merck
CN

TP0100

Sigma-Aldrich

Total Protein Kit, Micro

Synonym(s):

Bradford

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

Key Documents

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description

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Quality Level

100

parameter

18-26 °C temperature
50 μL sample volume

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Colorimetric, Endpoint
Suitable for protein determination for samples of up to 0.5 mg/ml.
Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.
Total protein kit, micro can be used to determine the concentration of proteins in solution.

Kit Components Also Available Separately

Product No.
Description
SDS
  • Protein Standard 5 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

132.8 °F

Flash Point(C)

56 °C

Regulatory Information

高风险级别生物产品--人源产品
危险化学品

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Certificates of Analysis (COA)

Lot/Batch Number
0000472652
0000472652
0000355995
0000355995
0000263809

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