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U0880

Uricase from Candida sp.

Name: Uricase from <I>Candida</I> sp.
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Synonym(s):

Urate: oxygen oxoreductase

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About This Item

CAS Number:

9002-12-4

UNSPSC Code:

12352204

PubChem Substance ID:

24900619

NACRES:

NA.54

EC Number:

1.7.3.3 (BRENDA, IUBMB)

MDL number:

MFCD00082126

Specific activity:

≥2 units/mg solid

Recombinant:

expressed in E. coli

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Properties

Quality Segment

200

recombinant

expressed in E. coli

form

lyophilized powder

specific activity

≥2 units/mg solid

mol wt

35 kDa by SDS-PAGE

storage temp.

−20°C

SMILES string

[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description

General description

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.

Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:

to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem/physiol Actions

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Physical form

Powder; contains citrate and sucrose

Other Notes

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c