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U7504

Sigma-Aldrich

UDP-Glucuronosyltransferase 1A7 Isozyme human

recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution, ≥0.15 units/mg protein

Synonym(s):

UDP-glycosyltransferase, UGT1A7

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About This Item

EC Number:
MDL number:
UNSPSC Code:
12161501
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recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous glycerol solution

specific activity

≥0.15 units/mg protein

concentration

2-20 mg/mL protein

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UGT1A7(54577)

General description

Greater than 80% of the activity was observed after the microsomes had undergone five freeze-thaw cycles.
UGT1A7 catalyzes the glucuronidation of phenolic xenobiotics.

Biochem/physiol Actions

A family of enzymes that detoxify and enhance the urinary excretion of a wide variety of xenobiotic and endogenous substrates by transferring glucuronic acid to sulfhydryl, hydroxyl, aromatic amino, or carboxylic acid groups.
The UDP-Glucuronosyltransferases (UGT) comprise a family of enzymes that detoxify and enhance the urinary excretion of a wide variety of xenobiotic and endogenous substrates by transferring glucuronic acid to sulfhydryl, hydroxyl, aromatic amino, or carboxylic acid groups.

Physical form

Microsomal fraction in 100 mM potassium phosphate, pH 7.4, 20% (v/v) glycerol, 0.1 mM EDTA, 1 mM DTT

Other Notes

One unit will transfer one nanomole of glucuronic acid from uridine-5′-diphosphoglucuronic acid to octyl gallate per minute at pH 7.5 at 37 °C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Cyril Bigo et al.
Drug metabolism reviews, 45(1), 34-47 (2013-01-22)
The recent progresses in molecular biology and pharmacology approaches allowed the characterization of a series of nuclear receptors (NRs) as efficient regulators of uridine diphosphate glucuronosyltransferase (UGT) genes activity. These regulatory processes ensure an optimized UGT expression in response to
Liam C Hunt et al.
The Journal of biological chemistry, 288(18), 13006-13021 (2013-03-16)
Exogenous hyaluronan is known to alter muscle precursor cell proliferation, migration, and differentiation, ultimately inhibiting myogenesis in vitro. The aim of the current study was to investigate the role of endogenous hyaluronan synthesis during myogenesis. In quantitative PCR studies, the
[Importance and practice of UGT1A1 polymorphisms].
Atsushi Watanabe et al.
Nihon rinsho. Japanese journal of clinical medicine, 70 Suppl 7, 475-479 (2013-01-29)
Eric Lévesque et al.
The Journal of pharmacology and experimental therapeutics, 345(1), 95-101 (2013-02-07)
Despite the importance of UDP-glucuronosyltransferase (UGT) 1A1*28 in irinotecan pharmacogenetics, our capability to predict drug-induced severe toxicity remains limited. We aimed at identifying novel genetic markers that would improve prediction of irinotecan toxicity and response in advanced colorectal cancer patients
Raimo Tuuminen et al.
Transplantation, 95(9), 1084-1091 (2013-03-08)
Ischemia-reperfusion injury (IRI) and allograft dysfunction remain as two of the major clinical challenges after heart transplantation. Here, we investigated the effect of donor treatment with simvastatin and methylprednisolone on microvascular dysfunction and immunomodulation during IRI in rat cardiac allografts

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