U8501
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast
Type X, lyophilized powder, ≥40 units/mg protein
Synonym(s):
UTP:α-D-glucose-1-phosphate uridylyltransferase
biological source
bakers yeast
Quality Level
type
Type X
form
lyophilized powder
specific activity
≥40 units/mg protein
composition
Protein, 30-60% modified Warburg-Christian
foreign activity
UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%
storage temp.
−20°C
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General description
Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.
Application
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
- in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
- to quantify Suc synthase (SUS) enzyme activity in rice
- to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes
Biochem/physiol Actions
Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.
Physical form
Lyophilized, sulfate-free powder containing citrate buffer salt
Other Notes
One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Ziying Huang et al.
Molecular biology reports, 38(7), 4291-4302 (2010-11-30)
OsUgp2, a rice UDP-glucose pyrophosphorylase gene, has previously been shown to preferentially express in maturing pollens and plays an important role in pollen starch accumulation. Here, a 1943 bp promoter fragment (P1943) of OsUgp2 was characterized by 5' deletion and
Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types
Kato T, et al.
Plant Protection Science, 10(4), 442-450 (2007)
Orelvis Portal et al.
Plant cell reports, 30(5), 913-928 (2011-02-01)
Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA
Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions
Michael L N, et al.
Nature Biotechnology, 862?869-862?869 (2015)
Wen-Ling Deng et al.
Molecular plant-microbe interactions : MPMI, 23(9), 1184-1196 (2010-08-07)
Bacterial galU coding for a uridine diphosphate-glucose pyrophosphorylase plays an important role in carbohydrates biosynthesis, including synthesis of lipopolysaccharides (LPS), membrane-derived oligosaccharides, and capsular polysaccharides. In this study, we characterized the galU mutant of Pseudomonas syringae pv. syringae 61 (Psy61)
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