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Merck
CN

UPS2

Sigma-Aldrich

Proteomics Dynamic Range Standard Set

Protein Mass Spectrometry Calibration Standard

Synonym(s):

Dynamic Range Standards, Proteomics Standard Set

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About This Item

EC Number:
232-650-8
UNSPSC Code:
12161503
NACRES:
NA.24

Key Documents

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biological source

human

Quality Level

200

form

ready-to-use solution

quality

Protein Mass Spectrometry Calibration Standard

concentration

10.6 μg/ampule protein

technique(s)

mass spectrometry (MS): suitable

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.

Application

Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins, embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes. It has also been used as a standard to spike HeLa cells for label-free quantification.
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation. It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.

Kit Components Also Available Separately

Product No.
Description
SDS
  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS

Signal Word

Danger

Hazard Statements

H315,H318,H334,H335,H360F

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

高风险级别生物产品--人源产品
低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
SLCR5904
SLCR5903
0000260162
SLCN1435
SLCM8684

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Jürgen Cox et al.
Molecular & cellular proteomics : MCP, 13(9), 2513-2526 (2014-06-20)
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination and normalization procedure called MaxLFQ that is fully
Mari J Aaltonen et al.
The Journal of cell biology, 213(5), 525-534 (2016-06-01)
Mitochondria exert critical functions in cellular lipid metabolism and promote the synthesis of major constituents of cellular membranes, such as phosphatidylethanolamine (PE) and phosphatidylcholine. Here, we demonstrate that the phosphatidylserine decarboxylase Psd1, located in the inner mitochondrial membrane, promotes mitochondrial
L Arike et al.
Journal of proteomics, 75(17), 5437-5448 (2012-07-10)
Three different label-free proteome quantification methods--APEX, emPAI and iBAQ--were evaluated to measure proteome-wide protein concentrations in the cell. All the methods were applied to a sample from Escherichia coli chemostat culture. A Pearson squared correlation of approximately 0.6 among the
Björn Schwanhäusser et al.
Nature, 473(7347), 337-342 (2011-05-20)
Gene expression is a multistep process that involves the transcription, translation and turnover of messenger RNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale.
Taejoon Kwon et al.
Journal of proteome research, 10(7), 2949-2958 (2011-04-15)
Shotgun proteomics using mass spectrometry is a powerful method for protein identification but suffers limited sensitivity in complex samples. Integrating peptide identifications from multiple database search engines is a promising strategy to increase the number of peptide identifications and reduce
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