UniScript^™ - Taq Labeling Kit

The UniScript - Taq Labeling Kit provides a convenient method for producing hapten-labeled single-stranded DNA probes from single or double-stranded templates. The easy one-step linear amplification-labeling procedure uses taq DNA polymerase and a single primer to incorporate hapten-modified dUTPs into a single stranded amplification product. A major advantage of this method is that sense or anti-sense probes can be prepared from any double stranded template without the need for RNA polymerase promoters. Probe sequence is entirely dictated by the user-designed primer. In addition, single-stranded DNA probes do not require denaturation prior to hybridization and are not susceptible to RNase degradation. Probes constructed with this kit are as sensitive in blotting applications as those generated by random-primed and in vitro transcription labeling methods. The kit has been optimized for producing labeled probes less than 1 kb in length utilizing Fluorescein-12-dUTP, Digoxigenin-11-dUTP or Biotin-16-dUTP (labeled deoxyribonucleotides not included).

UniScript is a trademark of Sigma-Aldrich Co. LLC