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V5140

Sigma-Aldrich

VPS34 human

recombinant, expressed in Sf9 cells

Synonym(s):

VPS34 human

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in Sf9 cells

Quality Level

form

liquid

specific activity

≥100 units/μg protein

mol wt

98.2 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

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General description

This Human VPS34 (vacuolar protein sorting 34) protein has a N-terminal FLAG-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

VPS34 was shown to be required for proper liver and heart function. Mice deficient in VPS34 develop hepatomegaly and cardiomegaly. [1]

Unit Definition

One unit will transfer one picomole of phosphate from ATP to a phospholipid mixture per minute at 30 °C at pH 7.4.

Physical form

Formulatedin a buffered solution consisting of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2mM KCl, 80 μg/ml FLAG peptide, 20% glycerol., and 3 mM DTT.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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