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Merck
CN

V5140

VPS34 human

recombinant, expressed in Sf9 cells

Synonym(s):

VPS34 human

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥100 units/μg protein
Recombinant:
expressed in Sf9 cells
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recombinant

expressed in Sf9 cells

Quality Segment

form

liquid

specific activity

≥100 units/μg protein

mol wt

98.2 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

General description

This Human VPS34 (vacuolar protein sorting 34) protein has a N-terminal FLAG-tag, expressed in Sf9 insect cells via a baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

VPS34 was shown to be required for proper liver and heart function. Mice deficient in VPS34 develop hepatomegaly and cardiomegaly.

Physical form

Formulatedin a buffered solution consisting of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2mM KCl, 80 μg/ml FLAG peptide, 20% glycerol., and 3 mM DTT.

Other Notes

One unit will transfer one picomole of phosphate from ATP to a phospholipid mixture per minute at 30 °C at pH 7.4.


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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