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V5764

Anti-Vesicular GABA Transporter antibody produced in rabbit

Name: Anti-Vesicular GABA Transporter antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-VGAT

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.77

MDL number:

MFCD02264204

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, WB

Citations:

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

potency

2-4 mg per mL effective concentration (determined by immunoblotting, using mouse brain and rat brain extracts)

mol wt

antigen ~55 kDa

species reactivity

rat, mouse

packaging

pkg of 200 μL

storage condition

avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)

concentration

~1.0 mg/mL

technique(s)

microarray: suitable, western blot: 2-4 μg/mL using mouse brain and rat brain extracts (S1 fraction)

UniProt accession no.

Q9H598
O35458
O35633

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SLC32A1(140679)
mouse ... Slc32a1(22348)
rat ... Slc32a1(83612)

General description

Vesicular GABA transporter (VGAT), also known as solute carrier family 32 member 1 (SLC32A1) or vesicular inhibitory amino acid transporter (VIAAT), is encoded by the gene localized on human chromosome 20q11.23. The encoded protein is highly expressed in both γ-aminobutyric acid -ergic (GABAergic) and glycinergic nerve endings.

Immunogen

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Application

Anti-Vesicular GABA Transporter antibody produced in rabbit has been used in:

immunohistochemistry
western blot
immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Vesicular GABA transporter (VGAT) plays a vital role in loading of presynaptic vesicles with glycine and γ-aminobutyric acid (GABA). VGAT is precisely involved in packing GABA into synaptic vesicles. In addition, it also has a crucial role in inhibitory synaptic transmission. Genetic variations in the gene leads to hyperekplexia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Forebrain Ischemia Triggers GABAergic System Degeneration in Substantia Nigra at Chronic Stages in Rats

Lin B

Cardiovascular Psychiatry and Neurology (2010)

Distribution and Posttranslational Modification of Synaptic ERα in the Adult Female Rat Hippocampus

Tabatadze N

Endocrinology, 154, 2013-2013 (2013)

Cyclin-Dependent Kinase 5 Is Involved in the Phosphorylation of Gephyrin and Clustering of GABAA Receptors at Inhibitory Synapses of Hippocampal Neurons

Kalbouneh H

PLoS ONE, 9 (2014)

Forebrain ischemia triggers GABAergic system degeneration in substantia nigra at chronic stages in rats.

B Lin et al.

Cardiovascular psychiatry and neurology, 2010, 506952-506952 (2010-10-29)

The long-term consequences of forebrain ischemia include delayed Parkinson's syndrome. This study revealed delayed neurodegeneration in the substantia nigra 8 weeks after 12.5 minutes of global ischemia in rat brain. Following neuronal loss of 30-40% in central and dorsolateral striatum

Dopaminergic neurons inhibit striatal output via non-canonical release of GABA

Tritsch NX

Nature, 490, 262-266 (2012)

View All Related Papers

Global Trade Item Number

SKU	GTIN
V5764-200UL	04061837481161

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