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Merck
CN

V6258

D-Val-Leu-Arg p-nitroanilide diacetate salt

≥95% (HPLC), suitable for ligand binding assays

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About This Item

Empirical Formula (Hill Notation):
C23H38N8O5 · 2 C2H4O2
CAS Number:
64816-14-4
Molecular Weight:
626.70
UNSPSC Code:
12352209
PubChem Substance ID:
329829059
NACRES:
NA.26
MDL number:
MFCD09250882

Key Documents

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Product Name

D-Val-Leu-Arg p-nitroanilide diacetate salt, ≥95% (HPLC)

SMILES string

CC(O)=O.CC(O)=O.N[C@H](C(C)C)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O

InChI key

WGVZWNUZAXIGKU-HYKWBEMSSA-N

InChI

1S/C23H38N8O5.2C2H4O2/c1-13(2)12-18(30-22(34)19(24)14(3)4)21(33)29-17(6-5-11-27-23(25)26)20(32)28-15-7-9-16(10-8-15)31(35)36;2*1-2(3)4/h7-10,13-14,17-19H,5-6,11-12,24H2,1-4H3,(H,28,32)(H,29,33)(H,30,34)(H4,25,26,27);2*1H3,(H,3,4)/t17-,18-,19+;;/m0../s1

assay

≥95% (HPLC)

form

powder

composition

Peptide content, ~70%

technique(s)

ligand binding assay: suitable

color

white

storage temp.

−20°C

Quality Level

200

Related Categories

Analysis Note

Salt content will vary.

Application

D-Val-Leu-Arg p-nitroanilide diacetate salt has been used as a substrate to determine the amidolytic activity of the fibrinolytic enzyme from Bacillus subtilis DC33. It has also been used as a substrate to determine the activity of fibrinolytic enzymes from the earthworm Eisenia fetida.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H361fd

Hazard Classifications

Repr. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCD2998
BCBW4461
BCBS5279V
BCBN3219V
BCBM1303V

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T L Miranda et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 28(5), 505-512 (1995-05-01)
Hydrolysis of D-valyl-L-leucyl-L-arginine p-nitroanilide (D-Val-Leu-Arg-Nan) at five different concentrations (10-20 microM) by human urinary kallikrein was studied in the absence and in the presence of increasing concentrations of basic pancreatic trypsin inhibitor (BPTI) (1.35-9.15 nM). The data indicate that the
M O Sousa et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 29(3), 327-334 (1996-03-01)
Hydrolysis of seven N alpha-substituted L-arginine 4-nitroanilides: benzoyl-arginine p-nitroanilide (Bz-Arg-Nan), tosyl-arginine p-nitroanilide (Tos-Arg-Nan), acetyl-leucyl-arginine p-nitroanilide (Ac-Leu-Arg-Nan), acetyl-phenylalanyl-arginine p-nitroanilide (Ac-Phe-Arg-Nan), benzoyl-phenylalanyl-arginine p-nitroanilide (Bz-Phe-Arg-Nan), tosyl-phenylalanyl-arginine p-nitroanilide (Tos-Phe-Arg-Nan), and D-valyl-leucyl-arginine p-nitroanilide (D-Val-Leu-Arg-Nan), and the N alpha-substituted L-arginine ester: benzoyl-arginine ethyl ester (Bz-Arg-OEt), by
Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi
Cheng Tao Wang
Journal of Industrial Microbiology & Biotechnology, 33, 750-758 (2006)
Increase in ovarian kallikrein activity during ovulation in the gonadotrophin-primed immature rat.
Espey LL, Tanaka N, Winn V, Okamura H.
J. Reprod. Fert., 87, 503-508 (1989)
I J Zeitlin et al.
The Journal of physiology, 543(Pt 1), 221-231 (2002-08-16)
It has been postulated that the mammary kinin system may play a role in modulating mammary blood flow. Until the present study, the local release of bradykinin (BK) or other kinin system constituents into the mammary vasculature had not been
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