W4125
Williams′ Medium E
With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Medium E, Williams E Medium, Williams’ E medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.
Application
Williams′ E medium has been used in culturing mice/rat primary hepatocytes.
Preparation Note
Formulated to contain 10.8 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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The Journal of nutritional biochemistry, 63, 19-26 (2018-10-14)
Trans-palmitoleic acid (trans-C16:1 n-7 or trans-Δ9-C16:1, TPA) is believed to improve several metabolic parameters according to epidemiological data. TPA may mainly come from direct intakes: however, data are inconsistent due to its very low amount in foods. Instead, TPA might
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Major urinary protein (MUP) 1 is a lipocalin family member abundantly secreted into the circulation by the liver. MUP1 binds to lipophilic pheromones and is excreted in urine. Urinary MUP1/pheromone complexes mediate chemical communication in rodents. However, it is unclear
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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