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Merck
CN

W4128

Sigma-Aldrich

Williams′ Medium E

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Williams’ E medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

Williams′ Medium E, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Williams′ Medium E has been shown to support the growth in long-term culture of adult liver epithelial cells.

Application

Williams′ Medium E has been used to culture primary human hepatocytes and to maintain hepatocellular carcinoma (JHH-6) cell lines.

Preparation Note

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
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Amino-acid coevolution can be referred to mutational compensatory patterns preserving the function of a protein. Viral envelope glycoproteins, which mediate entry of enveloped viruses into their host cells, are shaped by coevolution signals that confer to viruses the plasticity to
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Mammalian autophagy has been well characterized in the liver and in hepatocytes. Autophagy plays important roles in the normal physiology of the liver and in the pathogenesis of several liver diseases. This chapter will discuss the commonly used methods for
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Mitophagy is a lysosomal degradation pathway that selectively removes damaged, aged and dysfunctional mitochondria. Recent advances in understanding mitophagy highlight its importance in various physiological and pathological conditions including liver diseases. However, reliable quantitative assays to monitor mitophagy in cultured
Isolation and long-term cell culture of epithelial-like cells from rat liver.
G M Williams et al.
Experimental cell research, 69(1), 106-112 (1971-11-01)

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