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Y0396

Anti-YB-1 (C-terminal) antibody produced in rabbit

Name: Anti-YB-1 (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-BP-8, Anti-CSDA2, Anti-CSDB, Anti-DBPB, Anti-MDR-NF1, Anti-NSEP1, Anti-Y BOX binding protein, Anti-YBOX1

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells, western blot: 0.5-1 μg/mL using PC12 cell lysates

UniProt accession no.

P67809

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)

Description

General description

Y-box-binding protein (YB-1) also known as nuclease-sensitive element-binding protein 1 (NSEP1) is a member of the Cold-Sock Domain (CSD) containing proteins. It is composed of three domains: the N-terminal which is thought to function as a trans-activation regulatory domain, a highly conserved nucleic acid binding domain (the CSD), and C-terminal domain, involved in protein-protein interactions.

Immunogen

synthetic peptide corresponding to amino acids 307-324 of human YB-1, conjugated to KLH. The corresponding sequence is identical in rat and mouse YB-1 proteins.

Application

Anti-YB-1 (C-terminal) antibody has been used in

immunoblotting
immunoprecipitation
immunofluorescence
double-immunostaining

Biochem/physiol Actions

Anti-YB-1 (C-terminal) antibody is specific for YB-1 in rats, mice and humans. The immunizing peptide specifically inhibits the detection of the YB-1 band by immunoblotting.

Y-box-binding protein (YB-1) is shown to bind to the Y-box promoter element to regulate gene expression. It plays an important role in a wide variety of cellular functions such as transcriptional and translation regulation, DNA repair, drug resistance, and stress responses.

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

低风险生物材料

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000317391

0000185704

0000179735