Flow Cytometry Protocols: Methods in Molecular Biology, Vol. 91

This volume includes details on new methods that rely on recently developed instrumentation, without short-changing the time-tested standard techniques. Methods are given for detecting intracellular cytokines, nuclear antigens in leukemia and solid tumors, and cyclins. Protocols are included for performing functional studies like assessing viability, membrane potential, oxidative metabolism, drug resistance, and pharmacokinetics. Nucleic acid methods include cell cycle analysis, chromosome sorting, solid tumor ploidy analysis, apoptosis, and a flow cytometric application of FISH.