Antibody Engineering: Methods and Protocols

A collection of techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Included are procedures on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a variety of in vivo applications. Technologies are described for the characterization of antigen-binding affinity and specificity.