RNA Interference (RNAi): Nuts & Bolts of RNAi Technology

This book offers protocols and advice for the use of RNA interference for knocking down expression of target genes in a number of different eukaryotes. Varied applications in mammalian systems are explored in depth by authors currently working at the leading edge of RNA intererence methodologies. Chapters include: Design, synthesis and preparation of synthetic RNAi; Expression of RNAi from recombinant DNA and viral vectors; High-throughput RNAi applications; RNAi in mammalian systems.