Gene Cloning: Principles and Applications

The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Existing textbooks describe what has been learnt from cloned genes but include little detail on how the cloning was achieved and how the techniques involved are carried out in the laboratory. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions experimentally.