Chromobox protein homolog 3 (UniProt: Q13185; also known as HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein) is encoded by the CBX3 gene (Gene ID: 11335) in human. CBX3 is a major isoform of heterochromatin protein 1 family that recognizes and binds dimethylated and trimethylated histone H3 tails methylated at lysine 9, leading to epigenetic repression. It is reported to promote neuronal maturation, kidney development, differentiation of embryonic stem cells (ESC) to smooth muscle in culture, and embryonic arteriogenesis. Its deregulation has been reported to promote the development of human cancers. It promotes the proliferation, invasion, and tumorigenesis of glioblastoma cells in vitro and in vivo. CBX3 is localized in both euchromatin and heterochromatin (transiently) compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes. It is phosphorylated by PIM1 during interphase and may be hyper-phosphorylated during mitosis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Peng, W., et al. (2022). Oncogene, 41(22); 3051-3063; Huang, C., et al. (2017). Genes Dev. 31(3); 241-236).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1K5 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Chromobox protein homolog 3 (CBX3). It targets an epitope within 16 amino acids from the N-terminal half.

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Chromobox protein homolog 3 (CBX3).

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CBX3/HP1 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CBX3/HP1 in lysates from A549, NIH3T3, and PC12 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CBX3/HP1 in Human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CBX3/HP1 in A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.