Anti-CD55 Antibody, clone 1J12 ZooMAb^® Rabbit Monoclonal

Complement decay-accelerating factor (UniProt: P08174) is encoded by the CD55 (also known as CR, DAF) gene (Gene ID: 1604) in human. CD55 is a cell surface membrane protein associated in lipid microdomains, that acts as a complement regulatory protein. It is composed of four short consensus repeat (SCR) domains, a serine/threonine-rich region, and a glycosylphosphatidylinositol (GPI) anchor that attaches it to the cell membrane. This structure allows it to interact with complement proteins and prevents the formation of the membrane attack complex by dissociating C3/C5 convertases as well as preventing the assembly of C3/C5 convertases. CD55 is widely expressed on the surface of most blood cells, epithelial cells, and endothelial cells. It is also present in various tissues, providing broad protection against complement-mediated damage. It is essential in many organ systems including pregnancy for protection of the semiallogeneic fetus or for preventing uncontrolled infiltration by white cells in their pro- and/or anti-inflammatory functions. CD55 has been implicated in autoimmune disorders, complement hyperactivation, angiopathic thrombosis, and protein-losing enteropathy (CHAPLE), paroxysmal nocturnal hemoglobinuria (PNH), and cancer progression and is overexpressed in several types of cancer. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bharti, R., et al., (2022), Cancer Lett. 551, 215935)

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human CD55.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD55 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD55 in human lung tissue lysate.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD55 in one million human peripheral blood mononuclear cells (PBMC).

Affinity Binding Assay: A representative lot of this antibody bound with CD55 peptide with at least one thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD55 in human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1J12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CD55. It targets an epitope within 24 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.