Anti-HNRNPH1 Antibody, clone 1E14 ZooMAb^® Rabbit Monoclonal

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Heterogeneous nuclear ribonucleoprotein H (UniProt: P31943; also known as hnRNP H) is encoded by the HNRNPH1 (also known as HNRPH, HNRPH1) gene (Gene ID: ) in human. Heterogeneous nuclear ribonucleoprotein H1 (HNRNPH1) is a member of the hnRNP family of RNA-binding proteins that plays critical roles in RNA metabolism and gene expression regulation. It is primarily localized in the nucleus but can shuttle to the cytoplasm. HNRNPH1 contains RNA recognition motifs (RRMs) responsible for binding heterogeneous nuclear RNA (hnRNA), pre-mRNA, and mature mRNAs. It is involved in pre-mRNA processing, alternative splicing, RNA transport, and stabilization. HNRNPH1 regulates the expression of several genes, such as CD44 through splicing mechanisms, and participates in cell cycle regulation, stress granule formation, and axonal transport. Dysregulation of HNRNPH1 has been implicated in cancers, neurodegenerative diseases, and mitochondrial homeostasis, making it a potential therapeutic target. For instance, it is involved in regulating neuroprotective cold-shock protein RBM3 expression, autophagy via phase separation in colorectal cancer, and alternative splicing in germ cells. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Feng, S., et al., (2022), Nat. Commun, 13(1); 3588; Lin, JQ., et al., (2023), EMBO J. 42(14); e113168)

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human HNRNPH1.

Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected HNRNPH1 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected HNRNPH1 in HEK-293 cell lysate.
Immunocytochemistry Analysis: A 1:1,000 Dilution from a representative lot detected HNRNPH1 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HNRNPH1 in human small intestine tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1E14 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects HNRNPH1. It targets an epitope within 16 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.