material
glass column
manufacturer/tradename
Varian
parameter
0-350 °C temperature (isothermal or programmed)
technique(s)
gas chromatography (GC): suitable
L × O.D. × I.D.
6.0 ft (1.8 m) × 1/4 in. × 2.0 mm
matrix
100/120 SUPELCOPORT support
matrix active group
3% SP-2100 phase
column type
packed GC
compatibility
for use with Varian
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General description
GC columns are of two types; packed column and capillary column. Capillary column carries the gas through a central aperture which is unimpeded through the entire length of column. In general, capillary columns are made longer to produce more theoretical plates. This also helps in band dispersion eventually narrowing the peaks and resulting in high resolution.
Use a glass column for applications where high inertness is required, such as for the analysis of active analytes. All glass columns undergo a proprietary high temperature silanization to ensure inertness. This column fits Varian 3000, 4000, 6000, and Vista (FID, inj A to det A).
Application
Glass GC column packed with 3% SP-2100 on Supelcoport 100/120 was used for gas chromatographic analysis of synthetic esters of pregnenolone, during characterization of the lipoidal derivatives of pregnenolone which was prepared by incubation of the steroid with adrenal mitochondria.
Legal Information
Varian is a registered trademark of Varian Medical Systems, Inc.
signalword
Danger
hcodes
Hazard Classifications
STOT RE 1 Inhalation
target_organs
Lungs
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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S Mellon-Nussbaum et al.
The Journal of biological chemistry, 254(24), 12500-12505 (1979-12-25)
Using mass spectrometric, radioisotopic, chromatographic and chemical techniques, five fatty acid esters of 3 beta-hydroxy-5-pregnen-20-one (pregnenolone) have been identified as components of the lipoidal derivatives biosynthesized in vitro with bovine adrenal mitochondria. The five compounds are: pregnenolone arachidonate, pregnenolone linoleate
Scott RPW.
Introduction to Analytical Gas Chromatography (1997)
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