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Merck
CN

24056

Supelco

SP®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

Synonym(s):

SP-2560, 100M.20UM.25MM

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About This Item

UNSPSC Code:
41115710
eCl@ss:
32119290

Key Documents

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material

fused silica

Quality Level

100

Agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

313

df

0.20 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

100 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Application

The SP-2560 Capillary GC Columns meets the requirements as a USP G5 and AOAC® 2012.13,Ce 1-h05,996.06 designation. They are specifically designed for the detailed separation of geometric and positional (cis/trans) isomers of fatty acid methyl esters (FAMEs) in array of industries.

Select Applicatons:
  • Comparision study for the simultaneous resolution of all the fatty acids (cis/trans) generally found in partially hydrogenated vegetable oils.
  • Utilization in GCxGC for separation of trans fats including elaidic and vaccenic acids in several foods including butter, beef tallow, cheese, and milk.

Other Notes

We offer a variety of chromatography accessories including analytical syringes

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
297442-08
297442-07
297297-06
297297-05
297439-06

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Antonio Natalello et al.
Journal of agricultural and food chemistry, 67(16), 4472-4482 (2019-04-02)
This work investigated the effects of feeding whole pomegranate byproduct (WPB) to lambs on ruminal, liver, and intramuscular fatty acids (FA). Seventeen lambs, divided into two groups, were fed for 36 days with a cereal-based concentrate diet (CON) or with
B Schettino et al.
Journal of dairy science, 100(8), 6256-6265 (2017-06-12)
Chia seed (Salvia hispanica L.) is the greatest known plant source of n-3 α-linolenic acid. The present study evaluated the effects of 3 inclusion levels of chia seed [zero (control); low, 2.7% (CLow); and high, 5.5% (CHigh)] in diets of
Evaluation of the CP-Sil 88 and SP-2560 GC columns used in the recently approved AOCS official method Ce 1h-05: Determination of cis-, trans-, saturated, monounsaturated, and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC method.
Ratnayake WMN
Journal of the American Oil Chemists' Society, 83 (6), 475-488 (2006)
Adriana Campione et al.
Animals : an open access journal from MDPI, 10(4) (2020-04-05)
The hazelnut skin is waste biomass rich in bioactive compounds that may affect lipid rumen metabolism, ruminant performance, and products' quality. Therefore, we investigated the effect of dietary hazelnut skin on milk production and composition and on rumen fatty acids
Fatty acid composition of human erythrocyte membranes by capillary gas chromatography-mass spectrometry.
Alexander LR
Journal of Chromatography. B, Biomedical Applications, 342, 1-12 (1985)
View All Related Papers

Protocols

GC Analysis of a 37-Component FAME Mix on SP™-2560

A protocol for GC Analysis of a 37-Component FAME Mix on SP™-2560.

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

FAME Standard for Optimizing GC System Performance

AOCS and AOAC methods for determining the trans fatty acid composition of foods by gas chromatography require the use of a highly polar cyanosilicone capillary column to provide the best peak resolution attainable of the numerous geometric (cis & trans) and positional isomers.

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column

Chromatograms

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