47868
D-Biotin
analytical standard
Synonym(s):
Biotin, D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
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About This Item
InChI key
YBJHBAHKTGYVGT-ZKWXMUAHSA-N
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
SMILES string
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
grade
analytical standard
feature
standard type water soluble vitamin
packaging
ampule of 100 mg
technique(s)
HPLC: suitable, gas chromatography (GC): suitable
mp
231-233 °C (lit.)
application(s)
pharmaceutical (small molecule)
vitamins, nutraceuticals, and natural products
format
neat
storage temp.
2-8°C
General description
Application
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Is Product 47868, D-Biotin, suitable for cell culture?
Product 47868 D-Biotin, is not cell culture tested, and you should not consider it for cell culture use. The Supelco biotin is a chemical standard to be used in chromatographic methods. For cell culture, consider either the animal cell culture tested biotin (Product No. B4639), or the plant cell culture tested biotin (Product No. B3399).
How do I solubilize Product 47868, D-Biotin?
Per the chemicals encyclopedia published by the Royal Society of Chemistry, 13th ed, biotin is readily soluble in hot water or dilute alkali; it is insoluble in other common organic solvents. In our labs, the basic solutions may be Trizma base, 2M ammonium hydroxide, or 1N sodium hydroxide. You can put the biotin into water, and titrate it with base until it goes into solution.
What column do you recommend for the analysis of water soluble vitamins, including Biotin?
The Discovery C18 and Ascentis RP-Amide HPLC columns have been used for the separation of ten water soluble vitamins: Ascorbic acid, Biotin, Cyanocobalamin, Folic acid, Niacin, Niacinamide, Pantothenic acid, Pyridoxine, Riboflavin, and Thiamine. Contact Supelco Technical Service for details -- techservice@sial.com
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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