54801-U
Superdex® Gel Filtration Column
phase Superdex 200 HR10/300 GL, L × I.D. 30 cm × 10 mm, 13-15 μm particle size
L × I.D.
30 cm × 10 mm
matrix active group
Superdex 200 HR10/300 GL phase
particle size
13-15 μm
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General description
Superdex Peptide offers very high resolution when compared to other size exclusion chromatography medium in the molecular weight range of 100-7,000 Daltons, making it the column of choice for separating peptides. Superdex 75 is ideal for separating monomers and dimers of low molecular weight recombinant proteins and peptides. Superdex 200 is intended for analyses of larger proteins (e.g., monoclonal antibodies).
Superdex 75 HR10/300 GL and Superdex 200 HR10/300 GL are high-resolution gel filtration columns for high-performance analytical and semi-preparative applications involving μg to mg of sample. These columns also can be used in conventional HPLC and FPLC™ systems. HiLoad columns are laboratory-scale columns of preparative grade Superdex 75 and Superdex 200 media. They can be used with a wide variety of equipment, including single pump-based configurations and FPLC, HPLC, and pilot scale systems.
Superdex 75 HR10/300 GL and Superdex 200 HR10/300 GL are high-resolution gel filtration columns for high-performance analytical and semi-preparative applications involving μg to mg of sample. These columns also can be used in conventional HPLC and FPLC™ systems. HiLoad columns are laboratory-scale columns of preparative grade Superdex 75 and Superdex 200 media. They can be used with a wide variety of equipment, including single pump-based configurations and FPLC, HPLC, and pilot scale systems.
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Application
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. Various applications include protein purification, studies of complex formation, and screening of uncharacterized samples.
Features and Benefits
- High resolution over a wide range of molecular weight (100 to 600,000 Daltons)
- Rapid separations
- High physical and chemical stability
- Long column life
- Column design compatible with biological analytes
- Easy scale-up (HR columns to HiLoad columns)
- Pre-packed for convenience and reproducibility
Legal Information
FPLC is a trademark of Cytiva
Superdex is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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