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Merck
CN

58358

Supelco

SUPELCOSIL LC-18-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
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Product Name

SUPELCOSIL LC-18-DB HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm

Agency

suitable for USP L1

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

11.0% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide shorter retention, better peak shape, and higher efficiency for organic bases than can be obtained on other Type A silica reversed-phase columns.

Application

SUPELCOSIL LC-18-DB HPLC Column has been used for the separation of:
  • Analytes of pharmaceutical relevance during their analysis using LC in conjunction with principal component analysis (PCA).
  • Uracil in aqueous solutions during its separation by reversed phase high performance liquid chromatography equipped with ultraviolet-visible (UV-Vis) detection.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Regulatory Information

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Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: repeatability, reproducibility and correlation.
Visky D, et al.
Journal of Chromatography A, 977(1), 39-58 (2002)
Ce Li et al.
American journal of physiology. Gastrointestinal and liver physiology, 320(3), G380-G395 (2021-01-28)
Pancreatic ductal adenocarcinoma (PDAC) is highly metastatic and represents one of the deadliest forms of human cancers. Previous studies showed that activation of Yes-associated protein 1 (YAP1) plays a key role in malignant transformation in the pancreas. In this study
Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns using principal component analysis
Euerby MR and Petersson P
Journal of Chromatography A, 994(1-2), 13-36 (2003)
O Majid et al.
Therapeutic drug monitoring, 23(2), 163-168 (2001-04-11)
Recipients of organ transplants remain particularly dependent on prednisolone as part of their maintenance immunosuppression. Despite this, the pharmacokinetics of prednisolone have never been fully characterized in these patients, and consequently dosing remains empirical. Accurate monitoring of prednisolone, its primary
S L Bramer et al.
Journal of pharmaceutical and biomedical analysis, 26(4), 637-650 (2001-08-23)
An LC/MS/MS method for the simultaneous determination of cilostazol, a quinolinone derivative, and three active metabolites, OPC-13015, OPC-13213, and OPC-13217, in human plasma was developed and validated. Cilostazol, its metabolites, and the internal standard, OPC-3930 were extracted from human plasma

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