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Merck
CN

713-1025

HPLC Analysis of Proteins on TSKgel® Butyl-NPR

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: TSKgel Butyl-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (814947)
mobile phase: [A] 0.1 M phosphate buffer, pH 7.0 plus ammonium sulfate, 2.3 M to 0 M in 12 min [B & C] = 0.1 M phosphate buffer, pH 7.0 plus ammonium sulfate, 1.8 M to 0 M in 60 min
flow rate: 1 mL/min
detector: UV, 280 nm

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Analyte

Description
    α-Chymotrypsin
    Ribonuclease

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