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HPLC Analysis of Human Growth Hormone (hGH) on Discovery® BIO PolyMA-WAX

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm I.D., 5 μm particles (59602-U)
mobile phase: [A] 20 mM Tris-HCl, pH 8.0; [B] 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0
gradient: 5 to 70% B in 30 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 210 nm
injection: 10 mg hGH

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

This Figure shows the degradation products of human growth hormone (hGH) well-separated on a Discovery BIO PolyMA-WAX anion-exchange column. The difference between the resolved compounds is the conversion of protein amide(s) to carboxylate(s), demonstrating both the power of the ion-exchange technique, and the efficiency of the Discovery BIO PolyMA-WAX column.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Human Growth Hormone human, HGH, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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