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About This Item
UNSPSC Code:
41115711
material
glass
product line
TSKgel®
analyte chemical class(es)
proteins
manufacturer/tradename
Tosoh 14443
parameter
≤20% organic solvent, ≤3 M salt concentration, 1.0 mL/min flow rate, 20 bar max. pressure, 4-30 °C temperature
technique(s)
HPLC: suitable
L × I.D.
5 cm × 5 mm
matrix
hydroxylated methacrylic polymer
matrix active group
sulfated glycosaminoglycan phase
particle size
10 μm
operating pH
5.5-9.0
capacity
>1.8 mg/mL, resin adsorption capacity (antithrombin III)
separation technique
affinity
storage temp.
2-8°C
General description
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
The heparin ligand in TSKgel Heparin-5PW affinity columns can interact with sample molecules by cation exchange as well as affinity chromatography. Cation exchange interaction takes place through O-sulfate and N-sulfate groups. The elution strength is similar to TSKgel SP-5PW.
In vivo, heparin prevents blood clotting as it reacts with clotting agents. This tendency is taken advantage of by using heparin to separate blood clotting factors such as antithrombin III, Factor VII, Factor IX,etc., and anti-heparin proteins such as glycoproteins, endoglycosidase, hyaluronidase, lipases, growth factors, RNA polymerases, and other nucleic acid-binding proteins.
The heparin ligand in TSKgel Heparin-5PW affinity columns can interact with sample molecules by cation exchange as well as affinity chromatography. Cation exchange interaction takes place through O-sulfate and N-sulfate groups. The elution strength is similar to TSKgel SP-5PW.
In vivo, heparin prevents blood clotting as it reacts with clotting agents. This tendency is taken advantage of by using heparin to separate blood clotting factors such as antithrombin III, Factor VII, Factor IX,etc., and anti-heparin proteins such as glycoproteins, endoglycosidase, hyaluronidase, lipases, growth factors, RNA polymerases, and other nucleic acid-binding proteins.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Regulatory Information
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