821369
ToyoScreen® SP-650M Process Development Column
phase sulfonic acid, pkg of 6 × 5 mL, 65 μm particle size
material
aluminum seal
polypropylene and polyethylene frit
product line
ToyoScreen®
feature
counter ion Na+
analyte chemical class(es)
proteins
packaging
pkg of 6 × 5 mL
manufacturer/tradename
Tosoh 21369
parameter
1-5 mL/min flow rate
20 mL/min max. flow rate
3 bar max. pressure
technique(s)
LPLC: suitable
L × I.D.
3 cm × 14.6 mm
matrix
hydroxylated methacrylic polymer
matrix active group
sulfonic acid phase
particle size
65 μm
operating pH
2-13
capacity
50 g/L±10 adsorption capacity (lysozyme)
separation technique
cation exchange
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General description
Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.
Features and Benefits
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Legal Information
ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation
Regulatory Information
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