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Merck
CN

823430

ToyoScreen® AF-rProtein A HC-650F Process Development Column

phase rProtein A, pkg of 5 × 1 mL, 45 μm particle size

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About This Item

UNSPSC Code:
41106500

Key Documents

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material

aluminum seal
polypropylene and polyethylene frit

product line

ToyoScreen®

specific analyte(s)

IgG

packaging

pkg of 5 × 1 mL

manufacturer/tradename

Tosoh 23430

parameter

0.2-1 mL/min flow rate
3 bar max. pressure
4 mL/min max. flow rate

technique(s)

HPLC: suitable

L × I.D.

3 cm × 6.4 mm

matrix

hydroxylated methacrylic polymer

matrix active group

rProtein A phase

particle size

45 μm

operating pH

2-13

capacity

>70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time)
 >80 g/L adsorption capacity (IgG)(static)

separation technique

affinity

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General description

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

Features and Benefits

Clean in place with 0.1 M NaOH.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation

Regulatory Information

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