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Merck
CN

G000280

HPLC Analysis of Antihypertensive and Diuretic Drugs on Discovery® RP-Amide C16

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery RP-AmideC16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)
mobile phase: [A] methanol; [B] 25 mM potassium phosphate, dibasic, pH 7.0 (22:78, A:B)
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 254 nm
injection: 10 μL

suitability

application for HPLC

application(s)

clinical
pharmaceutical (small molecule)

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Atenolol
    Caffeine
    Hydrochlorothiazide

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