technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C18, 15cm × 4.6mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (95:5), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (50:50), 0.1% TFA in water:0.1% TFA in acetonitrile
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50μL carboxymethylated apohemoglobin trypic digest in 50 mM ammonium bicarbonate
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Chromatograms
application for HPLC
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