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Merck
CN

G002333

HPLC Analysis of Heterocyclic Amines (HCAs) on Discovery® HS F5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)
mobile phase: [A] water (10 mM ammonium acetate, pH 4.0 with acetic acid); [B] methanol (v/v); (15:85, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 5 μg/mL
sample: 50 μg/mL each in mobile phase

suitability

application for HPLC

application(s)

chemicals and industrial polymers

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
    2-Amino-3-methyl-3H-imidazo[4,5-f]quinoline

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