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Merck
CN

G002435

HPLC Analysis of Fentanyl Tranquilizer on Ascentis® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid): [B] acetonitrile; (67:33, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 215 nm
injection: 10 μL
sample: 20 μg/mL in mobile phase

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Fentanyl

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