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Merck
CN

G002465

HPLC Analysis of Xanthines on Ascentis® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] methanol; (94:6, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 220 nm
injection: 10 μL
sample: 50 μg/mL each in mobile phase

suitability

application for HPLC

application(s)

life science and biopharma
vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    7-Methylxanthine
    1-Methylxanthine
    1,7-Dimethylxanthine

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