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Merck
CN

G002637

HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: [A] 1% acetic acid in water; [B] methanol, (85:15, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 10 μL
sample: as indicated in 1% acetic acid in water

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Sulfanilamide 50 mg/mL
    Sulfacetamide 50 mg/mL
    Sulfadiazine
    Sulfathiazole
    Sulfamerazine 50 mg/mL
    Sulfamethizole

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