G002663
HPLC Analysis of Anticholinergic Drugs on Ascentis® RP-Amide
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile; (85:15, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 220 nm
injection: 10 μL
sample: 50 μg/mL each in mobile phase
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
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