G002992
HPLC Analysis of Ulcer Treatment Drugs on Ascentis® RP-Amide
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)
mobile phase: [A] 10 mM ammonium phosphate, pH 6.2 with phosphoric acid; [B] acetonitrile
gradient: 5 to 15% B in 10 min; to 55% B in 8 min; held for 5 min
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: UV, 220 nm
injection: 5 μL
sample: as indicated in 95:5 water:methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
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