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Merck
CN

G003054

HPLC Analysis of Phenolic Antioxidants on Ascentis® C8

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis C8, 15 cm x 4.6 mm I.D., 5 μm particles (581424-U)
mobile phase: [A] 5% acetic acid; [B] (50:50) methanol:acetonitrile
gradient: 30 to 100% B in 10 min; held for 5 min
flow rate: 2.0 mL/min
column temp.: 30 °C
detector: UV, 280 nm
injection: 10 μL
sample: as indicated in 50:50 acetonitrile:2-propanol

suitability

application for HPLC

application(s)

cleaning products
cosmetics
personal care
vitamins, nutraceuticals, and natural products

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Propyl gallate
    Ethoxyquin
    2′,4′,5′-Trihydroxybutyrophenone
    tert-Butylhydroquinone 10 μg/mL
    Nordihydroguaiaretic acid
    3-tert-Butyl-4-hydroxyanisole 10 μg/mL
    2,6-Di-tert-butyl-4-hydroxymethylphenol 10 μg/mL
    Octyl gallate 10 μg/mL
    Lauryl gallate 10 μg/mL
    3,5-Di-tert-butyl-4-hydroxytoluene, analytical standard
    See All (10)

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