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Merck
CN

G003307

HPLC Analysis of Barbiturates on Ascentis® C8

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis C8, 15 cm x 4.6 mm I.D., 5 μm particles (581424-U)
mobile phase: [A] methanol; [B] water (45:55, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 214 nm
injection: 5 μL
sample: 10 μg/mL each in 50:50 water:methanol

suitability

application for HPLC

application(s)

forensics and toxicology
pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Barbital
    Phenobarbital
    Aprobarbital
    Butabarbital
    Pentobarbital
    Secobarbital

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