G003713
HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Phenyl
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)
mobile phase: [A] 13 mM ammonium formate (pH 3.0 with concentrated formic acid): [B] acetonitrile; (80:20, A:B)
flow rate: 1 mL/min, split to the MS
column temp.: 35 °C
detector: MS, ESI (+) in selected ion recording (SIR) mode
injection: 5 μL
sample: as indicated in 80:20, 13 mM ammonium formate (pH 3.0 with concentrated formic acid):acetonitrile
suitability
application for HPLC
application(s)
food and beverages
pharmaceutical (small molecule)
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