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Merck
CN

G003715

HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)
mobile phase: [A] 0.1% ammonium acetate; [B] acetonitrile with 0.1% ammonium acetate; (80:20, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 353 nm
injection: 10 μL
sample: 50 μg/mL each in 80:20, water:methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Oxytetracycline
    Rolitetracycline

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