G003728
HPLC Analysis of Steroids on Ascentis® Si
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] hexane; [B] ethanol; (88:12, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 245 nm
injection: 10 μL
sample: 50 μg/mL in 85:15, hexane:2-propanol
suitability
application for HPLC
application(s)
clinical
vitamins, nutraceuticals, and natural products
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Cortisone
Prednisone
Prednisolone
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