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Merck
CN

G003750

HPLC Analysis of Caffeine and Metabolites on Ascentis® RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: [A] 0.1% ammonium acetate in water, pH 4.5 with acetic acid; [B] acetonitrile
gradient: 2 to 8% B in 3 min; to 20% B in 9 min; held for 2 min; to 2% B in 0.1 min; held for 0.9 min
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 275 nm
injection: 10 μL
sample: as indicated in 0.1% ammonium acetate in water (pH 4.5 with acetic acid)

suitability

application for HPLC

application(s)

clinical

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Uric acid 50 mg/mL
    Xanthine
    7-Methylxanthine
    1-Methyluric acid
    1-Methylxanthine 20 mg/mL
    Theobromine 30 mg/mL
    1,7-Dimethyluric acid
    1,7-Dimethylxanthine 50 mg/mL
    Theophylline
    β-(Hydroxyethyl)theophylline 50 mg/mL
    Caffeine
    See All (11)

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