technique(s)

LC/MS: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with provastin and atorvastin at 5 and 100 ng/mL
SPE tube/cartridge: Supel-Select HLB SPE, 30 mg/1 mL (54181-U)
condition: 0.5 mL methanol:acetonitrile (1:1, v/v); 0.5 mL DI water
sample addition: 0.5 mL
washing: 0.5 mL 5% methanol
elution: 0.5 mL methanol:acetonitrile (1:1, v/v)
column: Ascentis Express C18, 5 cm x 2.1 mm, 2.7 μm particles (53822-U)
eluate post-treatment: evaporate to dryness with nitrogen at 37 °C, reconstitute in 0.5 mL DI water
mobile phase: 0.1% acetic acid diluted in 60% methanol
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: ABI 3200 QT; ESI(+), MRM (423.3/321.3, 423.3/101.0, 557.3/397.2 and 557.3/453.4)
injection: 5 μL

suitability

application for LC-MS
application for SPE

application(s)

clinical