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Merck
CN

G004542

HPLC Analysis of Folinic Acid Enantiomers on Astec® CHIROBIOTIC® T (RP mode)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] 1% triethylamine acetate, pH 4.5; [B] ethanol; (50:50, A:B)
flow rate: 1 mL/min
column temp.: 20 °C
detector: UV, 254 nm
injection: 5 μL
sample: 1.25 mg/mL in methanol

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Folinic acid calcium salt hydrate, BioXtra, ≥99.0% (HPLC)

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