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Merck
CN

G004569

HPLC Analysis of FMOC-Phenylalanine Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (60:40, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 220 nm
injection: 5 μL
sample: 5 mg/mL in methanol

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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