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Merck
CN

G004584

HPLC Analysis of Chlorthalidone Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] 10 mM ammonium acetate, pH 4.1; [B] methanol; (80:20, A:B)
flow rate: 0.9 mL/min
column temp.: 25 °C
detector: UV, 254 nm
injection: 5 μL
sample: 2 mg/mL in methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Chlorthalidone, analytical standard

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